Home > Persephone User Guide > Run BLAST > TBLASTN

TBLASTN

Follow the steps below to run the TBLASTN program, which performs a protein versus translated nucleotide (all six frames) comparison.

  1. View the details form for some gene:
  2. Click the Protein tab.
  3. Click Run BLAST... The Run BLAST window displays.
  4. The Sequence text box will be filled with the protein sequence of the selected gene model. If needed, you can always paste your own sequence.


Note

The FASTAClosed header line is optional if only one sequence is used as a query. In case of multiple query sequences each of them should have the FASTA header. The identifier from the header will be shown in the Query column of the blast results window, so please keep the identifiers unique.

  1. In the Program panel check (select) the TBLASTN radio button.
  2. Select the genome(s) BLAST file to use from the map set tree panel.
  3. Check (select) the Fast or Normal radio button in the Sensitivity/Speed panel. (In the figure above Normal was selected.)

Note

The Primer selection is disabled.

  1. Optional. Check (select) the Override arguments in Advanced panel if the default parameters need modification. Please note, there is a "?" button that opens the help page with the list and description of BLAST parameters.
  2. Click OK to run. The BLAST in Progress window displays.
  3. When the BLAST process finishes, the BLAST Results window displays. See BLAST Results Window for details.