Persephone User Guide

The Blast Results window (see the figure below) is divided up into panels and tabs, which can vary depending on which BLAST program (BLASTP, BLASTX, BLASTN, or TBLASTN) you used.

These tabs and panels are listed in the table below. Click on a panel or tab name for more information.

Regardless of which BLAST program you used, a table with E-values and other associated information will be displayed on the right-hand side of the window.

The Copy Results to Clipboard button will copy the data grid onto the clipboard so it can be copied directly into an Excel spreadsheet.

To see the hits in a vertical map view, click either Display Map, which will display the matches on the corresponding map without closing the Blast Results window, or Display Map & Close, which will also display the results on the corresponding map but will close the Blast Results window

A new Real-time BLAST hits results column will be displayed on the right-side of the plate as shown in the example below. Please note that a BLAST track is created only for BLASTN and TBLASTN, when the subject is a genomic sequence and HSP can be shown using genomic coordinates in bp.

For BLASTP and BLASTX, the subject is a set of proteins and Persephone just highlights the corresponding gene without creating a new track.

If you click on the highlighted gene in the grid in the Maps tab, an Annotation Details window will open.

The BLAST results can be saved for future review. The button "Save Results" will create a record on the local disk that can be recalled via "Show Saved BLAST Results" button.

The last result is always saved temporarily. If not explicitly stored by pressing the button "Save Results", it will be overwritten with the next run of BLAST.